Celcyclus- en apoptosedetectiekit (groene Fluorescentie)

The Cell Cycle and Apoptosis Detection Kit (Green Fluorescence) is a kit that analyzes the cell cycle and apoptosis by detecting DNA content. After Cycle Green Reagent binds to nucleic acids, the fluorescence intensity (Ex /Em: 502/525 nm) is enhanced by 500 times, with high sensitivity, and the fluorescence intensity is directly proportional to the content of nucleic acids. After cells were stained with Cycle Green Reagent, assuming the fluorescence intensity of cells in the G0/G1 phase was 1, the theoretical fluorescence intensity of cells in the G2/M phase containing double DNA was 2, while the fluorescence intensity of cells in the S phase during DNA replication was between 1 and 2. In late apoptotic or necrotic cells, due to the shrinkage of the cell nucleus and DNA fragmentation, some genomic DNA is lost, so the fluorescence intensity is less than 1. In flow cytometry analysis, a sub-G1 peak, that is, the apoptotic peak, will appear.

Cells

• Cycle Green Reagent (1000X) • RNase A (100X)

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Gel pack with blue ice.

Stored at -20°C for 12 months, protected from light

Cell Cycle