Cell-Free Protein Synthesis Kit Pro

The Cell-Free Protein Synthesis Kit is a product for in vitro protein synthesis based on E. coli lysate. Coupled transcription/translation systems contain an RNA polymerase and the necessary cellular machinery needed to direct protein synthesis (e.g., ribosomes, translation factors and tRNAs) . Supplements such as amino acids, an energy source and NTPs complete the system. Proteins are expressed using DNA or RNA as templates. Cell-free protein expression, independent of viable cells, enables rapid, flexible and high-yield protein expression, with numerous advantages over traditional recombinant expression systems: 1. Time saving. Express target proteins in 1~2 hours, with peak yield achieved in 8~24 hours. 2. High protein expression level, up to more than 3 mg/ml. 3. Easy operation, Add DNA or RNA templates to the reaction system, and the reaction can be performed using 96-well plates or centrifuge tubes. Note: This product contains the chaperone proteins GroEL (~60 kDa) and GroES (~10 kDa), representing the most extensively characterized chaperone system currently under research. They create a closed folding chamber that provides an optimal environment for unfolded proteins, reducing misfolding and aggregation, thereby increasing the probability of soluble expression. However, protein solubility is influenced by multiple factors (e.g., inherent protein structure, expression system, reaction conditions, etc.) . While this product can significantly improve folding efficiency, soluble expression is not guaranteed for all proteins.

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• Cell-free system solution A Pro: 300 μl • Cell-free system solution B: 600 μl • CFPS-Control Plasmid: 2 μg

Store at -80°C. Valid for 12 months. Dry ice transportation. After opening, all components should be stored at -80°C. To avoid multiple freeze-thaw cycles, Solution A and Solution B can be aliquoted according to reaction volume, snap-frozen in liquid nitrogen, and then stored at -80°C.