Centrine 1 Antilichaam / CETN1

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Tissues where cilia are present, such as the retina and testis, express both Centrin-1 and -2, but Centrin-2 is also expressed in nondifferentiated, nonciliated retinal cells (retinoblastoma cells), liver, skeletal muscle, and cardiac muscle. In these tissues, Centrin associates with the centrosomes, mitotic spindle poles, and basal bodies. Knockdown studies reveal a requirement for Centrin in centriole duplication and organization of spindle pole morphology and the completion of cytokinesis. Centrin-3 plays a role in centrosome reproduction.

Immunohistochemistry (FFPE) : 1-2 µg/mL

Q12798

Human

A portion of amino acids 1-172 from human CETN1 protein was used as the immunogen for the Centrin 1 antibody.

CETN1/9880

IHC-P

Protein A/G affinity

Purified

This Centrin 1 antibody is available for research use only.

Aliquot the Centrin 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

1 mg/mL in 1X PBS; BSA free, sodium azide free

Optimal dilution of the Centrin 1 antibody should be determined by the researcher.

Cell projection, Cytoplasm, Nucleus

IHC staining of FFPE human testis tissue with Centrin 1 antibody (clone CETN1/9880) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.