CEP63 Antilichaam / Centrosomaal eiwit van 63 kDa

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

Q96MT8

Human

E.coli-derived human CEP63 recombinant protein (Position: Q294-D628) was used as the immunogen for the CEP63 antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This CEP63 antibody is available for research use only.

After reconstitution, the CEP63 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the CEP63 antibody should be determined by the researcher.

Western blot analysis of CEP63 using anti-CEP63 antibody. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP63 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Strong bands are detected at approximately 70 kDa and 49 kDa, consistent with the major endogenous CEP63 isoform (617 aa, ~71 kDa) and a known smaller isoform or proteolytic fragment. The predicted ~81 kDa full-length isoform is weak or absent, a pattern commonly reported for CEP63 due to low abundance and centrosomal processing.