Ch-TOG-antilichaam / CKAP5

Cytoskeleton-associated protein 5 is a microtubule-associated protein that in humans is encoded by the CKAP5 gene. It is mapped to 11p11.2. This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunocytochemistry/Immunofluorescence: 2-4 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

Q14008

Human, Mouse, Rat

Amino acids M1-L221 from the human protein were used as the immunogen for the Ch-TOG antibody.

WB, IHC-P, ICC/IF, Direct ELISA

Affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

This Ch-TOG antibody is available for research use only.

Store the Ch-TOG antibody at -20°C.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Ch-TOG antibody should be determined by the researcher.

Cytoplasmic

Western blot testing of human 1) K562, 2) HL60, 3) HEK293 and 4) PC-3 cell lysate with Ch-TOG antibody. Predicted molecular weight ~225 kDa.