Chk2 (CHEK2) Mouse Monoclonal Antibody [Kloon ID: LBI2F4]

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

Carrier-free (BSA/glycerol-free) CHEK2 mouse monoclonal antibody, clone LBI2F4

CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53

Homo sapiens checkpoint kinase 2 (CHEK2), transcript variant 1, mRNA.

11200

O96017

Unconjugated

Cell cycle, p53 signaling pathway

Cell cycle, p53 signaling pathway

WB 1:500~1000, IF 1:50~100, FLOW 1:100

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Druggable Genome, Protein Kinase, Stem cell - Pluripotency

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

CHEK2

https://www.ncbi.nlm.nih.gov/gene/?term=11200

https://www.uniprot.org/uniprot/O96017