CHO-K1 Lysaat

CHO-K1 cells are derived from the ovary of an adult Chinese hamster, often used in biological and medical research and commercially in the production of therapeutic proteins. They were introduced in the 1960s, are grown as a cultured monolayer and require the amino acid proline in their culture medium. CHO-K1 cells are used in studies of genetics, toxicity screening, nutrition and gene expression, particularly to express recombinant proteins. Today, CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics.

Hamster

WB

2 mg/mL (determined by BCA assay)

CHO-K1, ATGL0027-10 µg, ATGL0027-20 µg, ATGL0027-50 µg, ATGL0027-100 µg, ATGL0027-250 µg, ATGL0027-500 µg, ATGL0027-1 mg, ATGL0027-10, ATGL0027-20, ATGL0027-50, ATGL0027-100, ATGL0027-250, ATGL0027-500, ATGL0027-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Hamster

All lanes : CHO-K1 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody