CHP1 Antilichaam / Calcineurin B homologe proteïne 1

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

Q99653

Human, Mouse, Rat

A synthetic peptide corresponding to a sequence at the N-terminus of human CHP1 was used as the immunogen for the CHP1 antibody.

WB, ICC/IF, FACS

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This CHP1 antibody is available for research use only.

After reconstitution, the CHP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the CHP1 antibody should be determined by the researcher.

Nuclear, Perinuclear (Golgi), Cell membrane

Immunofluorescent staining of CHP1 using anti-CHP1 antibody (green) and anti-Beta Tubulin antibody (red) . CHP1 was detected in an immunocytochemical section of human A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-CHP1 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used. Nuclear and perinuclear staining observed in human cell lines.