CLIA Kit voor N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)

NT-Pro-BNP; ; N-BNP

P07634

Chemiluminescent immunoassay for Antigen Detection.

Endocrinology; Cardiovascular biology

The microplate provided in this kit has been pre-coated with an antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) level in the sample or standard.

2h, 40min

Serum, plasma, tissue homogenates and other biological fluids

13.7-10,000pg/mL

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV

The minimum detectable dose of this kit is typically less than 5.5pg/mL

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

48T, 96T, 96Tx5, 96Tx10, 96Tx100

This assay has high sensitivity and excellent specificity for detection of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) . No significant cross-reactivity or interference between N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and analogues was observed.

N-Terminal Pro-Brain Natriuretic Peptide

Double-antibody Sandwich

Sus scrofa; Porcine (Pig)