CLIC4 Antilichaam / Chloride intracellulair kanaal eiwit 4

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200, Immunoprecipitation: 1:50, Flow Cytometry: 1:50

Q9Y696

Human, Mouse, Rat

A synthesized peptide derived from human CLIC4 was used as the immunogen for the CLIC4 antibody.

30C24

WB, IHC, ICC, IF, IP, FACS

Affinity-chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This CLIC4 antibody is available for research use only.

Store the CLIC4 antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Optimal dilution of the CLIC4 antibody should be determined by the researcher.

Cytoplasmic, membranous

Western blot analysis of CLIC4 using anti-CLIC4 antibody. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLIC4 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for CLIC4 at approximately 29 kDa. The expected band size for CLIC4 is at 29 kDa.