Clusterin Antibody / CLU / APO-J

Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, 伪 and 尾, are associated through disulfide bonds. The 尾 subunit (also called ApoJ伪) corresponds to residues 23-227. The 伪 subunit (also called ApoJ尾) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences 尾-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis.

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

P10909

Human

A portion of amino acids 150-300 from human CLU protein was used as the immunogen for the Clusterin antibody.

CLU/4740

IHC-P, WB

Protein G affinity

Purified

This Clusterin antibody is available for research use only.

Aliquot the Clusterin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

1 mg/mL in 1X PBS; BSA free, sodium azide free

Optimal dilution of the Clusterin antibody should be determined by the researcher.

Cytoplasm, Secreted

IHC staining of FFPE human adrenal gland tissue with Clusterin antibody (clone CLU/4740) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.