CNTF

CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity. Recombinant human CNTF is synthesized as a 199 amino acid polypeptide (22.7 kDa) lacking a hydrophobic N-terminal signal for secretion

CNTF; HCNTF

1270

P26441

NP_000605

NM_000614

11q12.2

Human

Hamster, Mouse, Rat, Frog, Human

MAFTEHSPLT PHRRDLCSRS IWLARKLRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM

< 0.1 ng/µg of protein (< 1EU/µg)

98% by SDS-PAGE & HPLC analyses

Determined by its ability to stimulate proliferation of human TF-1 cells using an ED50 concentration range of 50-150 ng/ml.

199

Lyophilized

5 mM Sodium Phosphate, pH 7.5 + 75 mM NaCl

22.7 kDa

E. coli