Codon-geoptimaliseerd FLP Recombinase (FLPo) Adenovirus (Ad-CMV-FLPo)

FLP from Saccharomyces cerevisiae, a site-specific recombinases (SSR) from the λ integrase family, recognizes distinct 34 bp FRT sites. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe, with a 4-fold increase in recombination efficiency at 37C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin. FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency for both in vivo and in vitro in mouse. This adenovirus express FLPo under a control of a CMV promoter.

Codon-optimized FLP Recombinase (FLPo) Adenovirus

41106621

3822190000

Pre-Made

Dry Ice

-80C

12 Months

Ad-CMV-FLPo

Human Adenovirus Type5 (dE1/E3)

CMV (ubiquitous)

DMEM, 2% BSA, 2.5% Glycerol

1x10^10 PFU/ml