Codon-geoptimaliseerde FLP (FLPo) met mCherry Adenovirus (Ad-mCherry-FLPo)

FLP is a site-specific recombinases (SSR) from the λ integrase family, which recognizes distinct 34 bp FRT sites. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe, with a 4-fold increase in recombination efficiency at 37C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin. FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency in mouse cells. This adenovirus express both FLPo and mCherry as a fluorescent marker. FLPo and mCherry are driven by the same CMV promoter and separated by a 2A peptide.

Codon-optimized FLP (FLPo) with mCherry Adenovirus

41106621

3822190000

Pre-Made

Dry Ice

-80C

12 Months

Ad-mCherry-FLPo

Human Adenovirus Type5 (dE1/E3)

CMV (ubiquitous)

MCherry

DMEM, 2% BSA, 2.5% Glycerol

1x10^10 PFU/ml