COL3A1 Antilichaam / Collageen III

COL3A1, also called EDS4A or Collagen alpha-1 (III), is a protein that in humans is encoded by the COL3A1 gene. It is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1 (III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it is the major ligand of GPR56 in the developing brain. COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry (intracellular) : 1-3ug/million cells

P02461

Human, Mouse, Rat

Amino acids D1222-E1455 from the human protein were used as the immunogen for the COL3A1 antibody.

WB, IHC-P, FACS

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This COL3A1 antibody is available for research use only.

After reconstitution, the COL3A1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the COL3A1 antibody should be determined by the researcher.

Extracellular

IHC staining of FFPE human tonsil tissue with COL3A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.