COTL1 cDNA

COTL1 encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although COTL1 has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes.

CLP

16q24.1

Human

PATGen (puc19-derived cloning vector)

ATGGCCACCAAGATCGACAAAGAGGCTTGCCGGGCGGCGTACAACCTGGTGCGCGACGACGGCTCGGCCGTCATCTGGGTGACTTTTAAATATGACGGCTCCACCATCGTCCCCGGCGAGCAGGGAGCGGAGTACCAGCACTTCATCCAGCAGTGCACAGATGACGTCCGGTTGTTTGCCTTCGTGCGCTTCACCACCGGGGATGCCATGAGCAAGAGGTCCAAGTTTGCCCTCATCACGTGGATCGGTGAGAACGTCAGCGGGCTGCAGCGCGCCAAAACCGGGACGGACAAGACCCTGGTGAAGGAGGTCGTACAGAATTTCGCTAAGGAGTTTGTGATCAGTGATCGGAAGGAGCTGGAGGAAGATTTCATCAAGAGCGAGCTGAAGAAGGCGGGGGGAGCCAATTACGACGCCCAGACGGAGTAA

COTL1, CLP, COTL1, ATGD0208-10 µg, ATGD0208-20 µg, ATGD0208-50 µg, ATGD0208-100 µg, ATGD0208-250 µg, ATGD0208-500 µg, ATGD0208-1 mg, ATGD0208-10, ATGD0208-20, ATGD0208-50, ATGD0208-100, ATGD0208-250, ATGD0208-500, ATGD0208-1

Store the plasmid at -20C.

Lyophilized

Cytoskeleton

606748

NP_066972.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_021149.3

429bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.