Creatine kinase B-type Antilichaam / CKBB

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type) . There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL

P12277

Human, Mouse, Rat, Hamster, Guinea pig

A recombinant human full-length CKB protein was used as the immunogen for the Creatine kinase B-type antibody.

CKBB/6636

IHC-P, WB

Protein G affinity

Purified

This Creatine kinase B-type antibody is available for research use only.

Aliquot the Creatine kinase B-type antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the Creatine kinase B-type antibody should be determined by the researcher.

Cytoplasm

Western blot analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, Human Stomach, Mouse Stomach, Rat Stomach, Hamster Stomach and Guinea pig Stomach tissue lysates using Creatine kinase B-type antibody (clone CKBB/6636) . Predicted molecular weight ~43 kDa.