CREB1 Antilichaam / cAMP responselement-bindend eiwit 1

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

P16220

Human, Monkey, Mouse, Rat

E.coli-derived human CREB1 recombinant protein (Position: A7-R270) was used as the immunogen for the CREB1 antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This CREB1 antibody is available for research use only.

After reconstitution, the CREB1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the CREB1 antibody should be determined by the researcher.

Western blot analysis of CREB1 using anti-CREB1 antibody. Lane 1: human 293T whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. CREB1 western blot of human, monkey, and rodent samples shows a characteristic doublet: a lower band near ~43-45 kDa and an upper band at ~48-50 kDa. The upper species corresponds to post-translationally modified (phosphorylated) CREB1, which commonly runs above its predicted ~37 kDa.