CRISPRa dCas9-VPR Lentivirus
Kwaliteit
ISO Gecertificeerd
Levering
24-48 uur
Technische Specificaties
Lentivirus expressing dCas9 (D10A and H840A) fused to the VPR activator complex (VP64, p65, Rta), which enhances gene expression by recruiting transcriptional machinery. Unlike the dCas9-SAM system, dCas9-VPR does not require MS2-modified sgRNAs, simplifying delivery with a single fusion protein encoded in one vector. Using specific sgRNAs, the dCas9-VPR system can be targeted to promoter regions, enabling activation of virtually any gene of interest.
Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
Beschrijving
Lentivirus expressing dCas9 (D10A and H840A) fused to the VPR activator complex (VP64, p65, Rta), which enhances gene expression by recruiting transcriptional m
Specificaties
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