CRLF2-eiwit (C-human IgG1-Fc, menselijk)

CRLF2

CRL2, CRLF2Y, ILXR, TSLPR

C-human IgG1-Fc

< 1.0 EU per μg protein as determined by the LAL method.

> 95 % as determined by SDS-PAGE.

1.Recombinant Human CRLF2/TSLPR Protein, hFc Tag (Cat. No. 29749-H02H) captured on Protein A chip can bind Recombinant Human TSLP Protein (R127A, R130S), His Tag (Cat. No. 16135-H08H) with an affinity constant of 1.43 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 2.Loaded Recombinant Human CRLF2/TSLPR Protein (hFc Tag) (Cat. No. 29749-H02H) on proA Biosensor, can bind Recombinant Cynomolgus TSLP Protein (His Tag) (Cat. No. 90911-C08H) with an affinity constant of 2.250 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized Recombinant Human CRLF2 Protein (ECD, Fc Tag) (Cat: 29749-H02H) at 5 μg/mL (100 μL/well) can bind Recombinant Human TSLP Protein (R127A, R130S, His Tag), Biotinylated (Cat: 16135-H08H-B), the EC50 is 2.5-7.5 ng/mL (QC tested) . 4.Recombinant Human TSLP Protein (R127A, R130S, His Tag) (Cat: 16135-H08H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human CRLF2 Protein (ECD, Fc Tag) (Cat: 29749-H02H) with an affinity constant of 0.511 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

Met1-Lys231

The recombinant human CRLF2 consists of 447 amino acids and predicts a molecular mass of 50.91 kDa.

Human