CRYAB Antilichaam

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants.

1410

P02511

Human, Mouse, Rat

Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human CRYAB (NP_001876.1) .

Unconjugated

Primary Antibodies

Apoptosis, Cancer, Cell Cycle, Neuroscience, Signal Transduction

Affinity purification

U-87MG

Batch dependent

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

None

Blue Ice

Store at -20˚ C. Avoid freeze / thaw cycles.

Observed: 20kDa

IgG

WB: 1:500 - 1:2000

CRYAB

293T

A-549

Mouse brain

Mouse heart

Mouse kidney

Crystallin, alpha B

Homo sapiens

CRYAB, CRYA2, CTPP2, HSPB5, MFM2, CMD1II, CTRCT16, HEL-S-101

WB

Liquid