CTLA4 / CD152 Proteïne (hFc dag)

Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2) . CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg) .

Cd152 Protein; Ctla-4 Protein; Ctla4 Protein; Ly-56 Protein

NP_005205.2

Human

Human

HFc-Tag

See Datasheet send with the product

< 1.0 EU per μg of the protein as determined by the LAL method

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

1.Loaded Recombinant Human CTLA4 / CD152 Protein (Fc Tag) on ProA Biosensor, can bind Recombinant Human CD80 / B7-1 Protein (His Tag) with an affinity constant of 210 nM as determined in BLI assay (Sartorius Octet RED384) . 2.Immobilized Recombinant Human CTLA4 / CD152 Protein (Fc Tag) at 2 μg/mL (100 μL/well) can bind Anti-CTLA4 (BMS) Antibody, Human IgG1, the EC50 is 2-6 ng/mL. 3.Immobilized Recombinant Human CD86 / B7-2 Protein (His Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human CTLA4 / CD152 Protein (Fc Tag), the EC50 is 3.5-10.5 ng/mL.

368

Lyophilized or frozen liquid

PBS, pH 7.4

See Datasheet

50 kDa (glycosylated)

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

HEK 293 cells