CTLA4-eiwit (C-His, mens)
Kwaliteit
ISO Gecertificeerd
Levering
24-48 uur
Technische Specificaties
CTLA4
ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12
• HEK 293
C-His
< 1.0 EU per μg protein as determined by the LAL method.
1.Flow cytometric analysis of anti-CTLA4 CAR expression. 5e5 of anti-CTLA4 CAR-293 cells were stained with 100 μL of 1:50 (2 μL stock solution in 100 μL FACS buffer) of APC-conjugated human CTLA4 Protein (Site-Specific APC Conjugation, His Tag, Cat. No. 11159-H86H-SA) and negative control protein. Non-transduced 293 cells were used as a control (left) . APC signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of APC-conjugated CTLA4 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human CTLA4 Protein (Site-Specific APC Conjugation, His Tag, Cat. No. 11159-H86H-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) .
Lys 36-Phe 162
The protein has a predicted molecular mass of 17 kDa.
Human
Beschrijving
CTLA4-eiwit (C-His, mens) Beschikbaar in 100 µg. Bestel eenvoudig online met snelle levering.
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