CTLL-2 Lysaat

CTLL-2 cell line was a subclone of cytotoxic T-cells derived from a C57bl/6 mouse. CTLL-2 cells require IL-2 for growth and are used to assay for its presence in conditioned media. CTLL-2 cells are used also for the detection of many factors in cytokine assay.

Mouse

WB

2 mg/mL (determined by BCA assay)

CTLL2, ATGL0033-10 µg, ATGL0033-20 µg, ATGL0033-50 µg, ATGL0033-100 µg, ATGL0033-250 µg, ATGL0033-500 µg, ATGL0033-1 mg, ATGL0033-10, ATGL0033-20, ATGL0033-50, ATGL0033-100, ATGL0033-250, ATGL0033-500, ATGL0033-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Mouse

All lanes : CTLL-2 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody