CXCR4 Antilichaam

CXCR4 signaling plays an important role in the migration, proliferation and quiescence of hematopoietic stem cell and their retention within the bone marrow, where it has high levels of SDF-1/CXCL12 (2) . It has been demonstrated that CXCR4 signaling mediates CD20 up-regulation on B cells (3) . CXCR4 is highly expressed in more than 23 types of cancer, including breast cancer, ovarian cancer, melanoma, and prostate cancer, while there is very less or no expression of CXCR4 in healthy tissues. CXCR4 expression in cancer cells has been reported to be associated with tumor survival, growth and metastasis in tissues with high levels of SDF-1/CXCL12, such as lungs, liver and bone marrow (4,5) . CXCR4 has been shown to regulate neuronal migration, cell positioning and axon wiring (6,7) . CXCR4 mutant mice displayed aberrant neuronal distribution, which implicates the role in neuronal disorders such as epilepsy. CXCR4 is also involved in WHIM syndrome (8) . WHIM mutations in CXCR4 were recently found in patients with Waldenstrom's macroglobulinemia, and these mutations are correlated to clinical resistance to ibrutinib (9,10) .

7852

P61073

NP_003458

Human, Mouse, Rat

1012

Unconjugated

Primary Antibodies

Independent Antibody Validation (Figure 2) shows similar CXCR4 expression profile in both human and mouse cell lines detected by two independent anti-CXCR4 antibodies that recognize different epitopes, 1009 against N-terminus ectodomain and 1012 against the second extracellular loop. CXCR4 proteins are detected in most of the cell lines, but not in THP-1 cells by the two independent antibodies. Additionally, Figure 2 shows the mouse CXCR4 protein in NIH/3T3 and C2C12 cell lines migrates slightly slower than human isoform b detected by both CXCR4 antibodies (1009 and 1012), which is well correlated with their calculated molecular masses (40.4 kDa vs 39.7 kDa) . Independent Antibody Validation1009 1012 siRNA knockdown validation (Figure 3) : Anti-CXCR4 antibody (1012) specificity was further verified by CXCR4 specific siRNA knockdown. CXCR4 signal in HeLa cells transfected with CXCR4 siRNAs was weaker in comparison with that in HeLa cells transfected with control siRNAs. siRNA knockdown validation1012 Animal Species Reactivity (Figure 4) : Anti-CXCR4 antibodies (1009 and 1012) can detect the expression of CXCR4 protein in rat thymus, but not in rat brain and rat heart. Animal Species Reactivity10091012

Chemokines & Cytokines, Stem Cell, Infectious Disease

CXCR4 Antibody is Protein A purified.

Cat. No. 1201 - HeLa Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (86%), Sheep: (86%)

CXCR4 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

CXCR4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human CXCR4 has four isoforms, including isoform a (356aa, 40.2kD), isoform b (352aa, 39.7kD), isoform c (423aa, 47.6kD), and isoform d (385aa, 43.4kD) . This antibody detects human isoform b only, but not other human isoforms. Mouse CXCR4 has two isoforms, including isoform 1 (359aa, 40.4kD) and isoform 2 (357aa, 40.2kD) . Rat CXCR4 has only one isoform identified so far (349aa, 39.4kD) .

CXCR4

Cat. No. 1202 - A431 Cell Lysate

Cat. No. 1210 - 293 Cell Lysate

Cat. No. 1211 - HepG2 Cell Lysate

Cat. No. 1282 - NIH/3T3 Cell Lysate

Cat. No. 1471 - Rat thymus Lysate

Chemokine (C-X-C motif) receptor 4

Homo sapiens

Dimitrov DS. Cell 1997;91:721-730

Chen et al. Circ Res. 2010;107:1083-93

Pavlasova et al. Blood 2016; 128: 1609-13

Sun et al. Cancer Metastasis Reviews 2010;29:709-22

Balkwill F. Nature Reviews 2004;4:540-50

Bagri et al. Development 2002; 129:4249-60

Yang et al. Development 2013;140:4554-64

Balabanjian et al. The Journal of Clinical Investigation 2008; 118:1074-84

Hunter et al. Blood 2014;123:1637-46

Treon et al. N. Engl. J. Med. 2015;372:1430-40

20197403

11799176

16638848

29954928

21309736

22039399

19525448

17515952

16825610

Schabath et al. CD24 affects CXCR4 function in pre-B lymphocytes and breast carcinoma cells. J Cell Sci. 2006;119 (Pt 2) :314-25. PMID: 16390867

CXCR4 Antibody: FB22, HM89, LAP3, LCR1, NPYR, WHIM, CD184, LESTR, NPY3R, NPYRL, HSY3RR, NPYY3R, D2S201E

ELISA, WB, IHC, IF

4503175

Liquid