Cycline D1 Antilichaam

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t (11; 14) (q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative) . Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Immunohistochemistry (FFPE) : 1-2 µg/mL

P24385

Human

A portion of amino acids 100-295 from human Cyclin D1 protein was used as the immunogen for the Cyclin D1 antibody.

CCND1/12039

IHC-P

Protein A/G affinity

Purified

This Cyclin D1 antibody is available for research use only.

Aliquot the Cyclin D1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

1 mg/mL in 1X PBS; BSA free, sodium azide free

Optimal dilution of the Cyclin D1 antibody should be determined by the researcher.

Nucleus

IHC staining of FFPE human thyroid tissue with Cyclin D1 antibody (clone CCND1/12039) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.