Cycline D1 Antilichaam

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t (11; 14) (q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative) . Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Immunohistochemistry (FFPE) : 1-2 µg/mL

P24385

Human

A portion of amino acids 100-295 from human Cyclin D1 protein was used as the immunogen for the Cyclin D1 antibody.

CCND1/12039

IHC-P

Protein A/G affinity

Purified

This Cyclin D1 antibody is available for research use only.

Aliquot the Cyclin D1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the Cyclin D1 antibody should be determined by the researcher.

Nucleus

IHC staining of FFPE human thyroid tissue with Cyclin D1 antibody (clone CCND1/12039) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.