Cycline E1 (CCNE1) (NM 001238) Humaan Recombinant eiwit

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016]

Purified recombinant protein of Human cyclin E1 (CCNE1), transcript variant 1, Ile96-Ala378, with N-terminal His tag, expressed in E. coli, 50ug

CCNE; pCCNE1

898

P24864

NM_001238

19q12

E. coli

N-His

Cell cycle, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer

Cell cycle, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer

>50 ug/mL as determined by microplate BCA method

> 80% as determined by SDS-PAGE and Coomassie blue staining

Liquid

50mM Tris, 8M Urea, pH8.0.

Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors

32.9 kDa

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

CCNE1

Human

NP_001229

https://www.ncbi.nlm.nih.gov/gene/898

https://www.uniprot.org/uniprot/P24864

https://www.ncbi.nlm.nih.gov/nuccore/NM_001238

https://www.ncbi.nlm.nih.gov/nuccore/NP_001229