CYCS cDNA

CYCS encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.

CYC, HCS, THC4, Cytochrome c

7p15.3

Human

PATGen (puc19-derived cloning vector)

ATGGGTGATGTTGAGAAAGGCAAGAAGATTTTTATTATGAAGTGTTCCCAGTGCCACACCGTTGAAAAGGGAGGCAAGCACAAGACTGGGCCAAATCTCCATGGTCTCTTTGGGCGGAAGACAGGTCAGGCCCCTGGATACTCTTACACAGCCGCCAATAAGAACAAAGGCATCATCTGGGGAGAGGATACACTGATGGAGTATTTGGAGAATCCCAAGAAGTACATCCCTGGAACAAAAATGATCTTTGTCGGCATTAAGAAGAAGGAAGAAAGGGCAGACTTAATAGCTTATCTCAAAAAAGCTACTAATGAGTAA

CYCS, CYC, HCS, THC4, CYCS, ATGD0213-10 µg, ATGD0213-20 µg, ATGD0213-50 µg, ATGD0213-100 µg, ATGD0213-250 µg, ATGD0213-500 µg, ATGD0213-1 mg, ATGD0213-10, ATGD0213-20, ATGD0213-50, ATGD0213-100, ATGD0213-250, ATGD0213-500, ATGD0213-1

Store the plasmid at -20C.

Lyophilized

Cancer

123970

NP_061820.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_018947.5

318bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.