CYP2C9 Antilichaam / Cytochroom P450 2C9

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24.

Immunohistochemistry (FFPE) : 1-2 µg/mL

P11712

Human

A portion of amino acids 1-200 from the human protein was used as the immunogen for the CYP2C9 antibody.

CYP2C9/13121

IHC-P

Protein G affinity

Purified

This CYP2C9 antibody is available for research use only.

Aliquot the CYP2C9 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the CYP2C9 antibody should be determined by the researcher.

Cytoplasm

IHC staining of FFPE human hepatocellular carcinoma tissue with CYP2C9 antibody (clone CYP2C9/13121) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.