Cytochroom P450 2A6 Polyklonale Antilichaam, APC-Cy7 Vervoeging

P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds (1) . Several P450 enzymes have been classified by sequence similarities as members of the CYP1A and CYP2A subfamilies (2) . NADPH cytochrome P450 reductase is a microsomal enzyme responsible for the transfer of electrons from NADPH to cytochrome P450 enzymes during the P450 catalytic cycle (3,4) . NADPH cytochrome P450 reductase is localized to the endoplasmic reticulum where it is also able to transfer electrons to heme oxygenase and cytochrome b5 (5,6) . NADPH cytochrome P450 reductase is structurally related to two separate flavoprotein families, ferredoxin nucleotide reductase (FNR) and flavodoxin (7) . Electron transfer of NADPH cytochrome P450 reductase requires the binding of two flavin cofactors, FAD and FMN, to the FNR and flavodoxin domains, respectively (8) .

Coumarin 7 hydroxylase; Coumarin 7-hydroxylase; CP2A6_HUMAN; CPA6; CYP2A; CYP2A3; CYP2A6; CYPIIA6; Cytochrome P450 2A6; Cytochrome P450 IIA3; Cytochrome P450 subfamily IIA phenobarbital inducible polypeptide 6; Cytochrome P450I; Cytochrome P450, family 2 subfamily A polypeptide 6; Flavoprotein linked monooxygenase; P450C2A; P450PB; Xenobiotic monooxygenase.

1548

Cytoplasm, Cell membrane

388-494/494

Cytochrome P450 2A6

APC-Cy7

KLH conjugated synthetic peptide derived from human CYP2A/Cytochrome P450 2A6

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purified by Protein A.

650nm/780nm

1µg/µl

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Unmodified

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

1548

Human, Mouse, Rat, Dog, Cow, Sheep