Danre Lmo2 Antilichaam / Rhombotin 2

Lmo2 does not directly bind to DNA but functions as a scaffold protein, forming complexes with other transcription factors to regulate gene expression. During zebrafish embryogenesis, Lmo2 is expressed in the intermediate cell mass, the site of primitive blood cell formation, and in the developing vasculature. It is required for the emergence of both erythroid and myeloid lineages, as well as for the proper development of endothelial cells. Loss of Lmo2 function in zebrafish results in severe defects in blood cell formation and vascular patterning, highlighting its central role in mesodermal differentiation. Because of its strong evolutionary conservation and defined expression patterns, zebrafish Lmo2 serves as a powerful model for studying hematopoiesis, angiogenesis, and transcriptional networks involved in early development. Lmo2 is also relevant to human health, as misregulation of Lmo2 has been associated with leukemia. This makes zebrafish Lmo2 a valuable tool in both basic research and disease modeling related to blood development and cancer biology.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q9PTJ3

Zebrafish

E. coli-derived Danre Lmo2 recombinant protein (amino acids M1-V159) was used as the immunogen for the Danre Lmo2 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Danre Lmo2 antibody is available for research use only.

After reconstitution, the Danre Lmo2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Danre Lmo2 antibody should be determined by the researcher.

Nucleus, Cytoplasm, Cell surface

IHC staining of FFPE zebrafish spinal cord tissue with Danre Lmo2 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.