DARPP 32 (fosfo Thr75) Antilichaam

DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.

360616

Q6J4I0

Q6J4I0

Human, Mouse

DARPP-32 (Thr75) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr75 of DARPP-32. The sequence of the immunogen is identical in human and mouse.

Unconjugated

Primary Antibodies

Neuroscience, Phospho-Specific

Affinity Purified

Batch dependent

Phospho-Specific

Blue Ice

For long term storage -80˚ C is recommended, but shorter term storage at -20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20˚ C.

32

DARPP 32 antibody is specific for the ~34k DARPP-32 phosphorylated at Thr75.

Applications include Dot Blots (DB) and Western Blots (WB) . Suitability for Immunohistochemistry (IHC) has not yet been determined. Rabbit anti-DARPP-32 (Thr75) recognizes human and mouse forms of the protein. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Ppp1r1b

Protein phosphatase 1, regulatory (inhibitor) subunit 1B

Rattus norvegicus

Svenningsson, P., Tzavara, E.T., Carruthers, R., Rachleff, I., Wattler, S., Nehls, M., McKinzie, D.L., Fienberg, A.A., Nomikos, G.G.and Greengard, P., "Diverse psychotomimetics act through a common signaling pathway, " Science, 302 (2003) 1412 - 1415.

Lindskog, M., Svenningsson, P., Pozzi, L., Kim, Y., Fienberg, A.A., Bibb, J.A.., Fredholm, B.B., Nairn, A.C., Greengard, P. and Fisone, G., "Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine, Nature (London), 418 (2002) 774 - 778.

Maldve, R.E., Zhang, T.A., Ferrani-Kile, K., Schreiber, S.S., Lippmann, M.J., Snyder, G.L., Feinberg, A.A., Leslie, S.W., Gonzales, R.A., and Morrisett, R.A., "DARPP-32 and the regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens, " Nature Neurosci. 5 (2002) 641 - 648.

Greengard, P., "The neurobiology of slow synaptic transmission, " Science 294 (2001) 1024 -1030.

WB

81884861

Liquid