DCAF7 cDNA

DCAF7, also known as DDB1-and CuL4-associated factor, involved in craniofacial development. This protein acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches. This protein associates with DIAPH1 and controls GLI1 transcriptional activity. This protein could be involved in normal and disease skin development.

AN11, HAN11, SWAN-1, WDR68

17q23.3

Human

PATGen (puc19-derived cloning vector)

ATGTCCCTGC ACGGCAAACG GAAGGAGATC TACAAGTATG AAGCGCCCTG GACAGTCTAC GCGATGAACT GGAGTGTGCG GCCCGATAAG CGCTTTCGCT TGGCGCTGGG CAGCTTCGTG GAGGAGTACA ACAACAAGGT TCAGCTTGTT GGTTTAGATG AGGAGAGTTC AGAGTTTATT TGCAGAAACA CCTTTGACCA CCCATACCCC ACCACAAAGC TCATGTGGAT CCCTGACACA AAAGGCGTCT ATCCAGACCT ACTGGCAACA AGCGGTGACT ATCTCCGTGT GTGGAGGGTT GGTGAAACAG AGACCAGGCT GGAGTGTTTG CTAAACAATA ATAAGAACTC TGATTTCTGT GCTCCCCTGA CCTCCTTTGA CTGGAATGAG GTGGATCCTT ATCTTTTAGG TACCTCAAGC ATTGATACGA CATGCACCAT CTGGGGGCTG GAGACAGGGC AGGTGTTAGG GCGAGTGAAT CTCGTGTCTG GCCACGTGAA GACCCAGCTG ATCGCCCATG ACAAAGAGGT CTATGATATT GCATTTAGCC GGGCCGGGGG TGGCAGGGAC ATGTTTGCCT CTGTGGGTGC TGATGGCTCG GTGCGGATGT TTGACCTCCG CCATCTAGAA CACAGCACCA TCATTTACGA AGACCCACAG CATCACCCAC TGCTTCGCCT CTGCTGGAAC AAGCAGGACC CTAACTACCT GGCCACCATG GCCATGGATG GAATGGAGGT GGTGATTCTA GATGTCCGGG TTCCCTGCAC ACCTGTCGCC AGGTTAAACA ACCATCGAGC ATGTGTCAAT GGCATTGCTT GGGCCCCACA TTCATCCTGC CACATCTGCA CTGCAGCGGA TGACCACCAG GCTCTCATCT GGGACATCCA GCAAATGCCC CGAGCCATTG AGGACCCTAT CCTGGCCTAC ACAGCTGAAG GAGAGATCAA CAATGTGCAG TGGGCATCAA CTCAGCCCGA CTGGATCGCC ATCTGCTACA ACAACTGCCT GGAGATACTC AGAGTGTAG

DCAF7, AN11, HAN11, SWAN-1, WDR68, DCAF7, ATGD0100-10 µg, ATGD0100-20 µg, ATGD0100-50 µg, ATGD0100-100 µg, ATGD0100-250 µg, ATGD0100-500 µg, ATGD0100-1 mg, ATGD0100-10, ATGD0100-20, ATGD0100-50, ATGD0100-100, ATGD0100-250, ATGD0100-500, ATGD0100-1

Store the plasmid at -20C.

Lyophilized

Development

605973

NP_005819.3

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_005828.4

1029bp

This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.