DCTN5 cDNA

DCTN5 encodes a subunit of dynactin, a component of the cytoplasmic dynein motor machinery involved in minus-end-directed transport. The encoded protein is a component of the pointed-end subcomplex and is thought to bind membranous cargo. A pseudogene of this gene is located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

16p12.2

Human

PATGen (puc19-derived cloning vector)

ATGGAGTTGG GCGAGCTGCT CTACAACAAG TCTGAGTACA TCGAGACGGC ATCTGGGAAC AAAGTCAGTC GCCAGTCAGT GTTGTGTGGA AGCCAGAACA TCGTTCTCAA TGGCAAGACC ATTGTGATGA ATGACTGTAT TATCCGAGGG GATCTGGCAA ATGTAAGAGT TGGACGTCAT TGTGTTGTGA AAAGTCGTAG TGTCATAAGG CCACCATTCA AGAAGTTCAG CAAAGGTGTT GCATTCTTTC CTTTACATAT TGGAGACCAT GTCTTTATTG AGGAAGATTG TGTGGTCAAC GCAGCACAGA TTGGTTCCTA TGTTCATGTT GGGAAGAACT GTGTGATTGG GCGCCGATGT GTGTTGAAAG ACTGCTGCAA AATTCTTGAC AACACAGTAT TACCTCCAGA AACTGTGGTT CCACCATTCA CTGTCTTCTC AGGCTGCCCA GGACTCTTCT CAGGGGAGCT CCCGGAGTGC ACTCAGGAGC TGATGATTGA CGTCACCAAG AGCTACTACC AGAAGTTTTT GCCCCTGACG CAAGTCTAG

DCTN5, DCTN5, ATGD0120-10 µg, ATGD0120-20 µg, ATGD0120-50 µg, ATGD0120-100 µg, ATGD0120-250 µg, ATGD0120-500 µg, ATGD0120-1 mg, ATGD0120-10, ATGD0120-20, ATGD0120-50, ATGD0120-100, ATGD0120-250, ATGD0120-500, ATGD0120-1

Store the plasmid at -20C.

Lyophilized

Signal Transduction

612962

NP_115875.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_032486.3

549bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.