DDDK-Man Rabbit mAb (AMR13114N)

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DDDDK-Tag Rabbit mAb (AMR13114N) .

DDDDK; DDDDK tag; DDDDK-tag

WB (Arabidopsis thaliana, Mus musculus, Homo sapiens, Other, Oreochroms mossambcus) IP (Arabidopsis thaliana, Homo sapiens, Mus musculus, Other) IHC (Mus musculus) IF (Mus musculus) ChIP (Arabidopsis thaliana)

WB 1:500 - 1:2000 IHC 1:50 - 1:500 IF 1:50 - 1:500 IP 1:50 - 1:500

Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Observed MW: Refer to Figures

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

DYKDDDDK