DDX56 cDNA

DDX56 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

DDX21, DDX26, NOH61

7p13

Human

PATGen (puc19-derived cloning vector)

ATGGAGGACTCTGAAGCACTGGGCTTCGAACACATGGGCCTCGATCCCCGGCTCCTTCAGGCTGTCACCGATCTGGGCTGGTCGCGACCTACGCTGATCCAGGAGAAGGCCATCCCACTGGCCCTAGAAGGGAAGGACCTCCTGGCTCGGGCCCGCACGGGCTCCGGGAAGACGGCCGCTTATGCTATTCCGATGCTGCAGCTGTTGCTCCATAGGAAGGCGACAGGTCCGGTGGTAGAACAGGCAGTGAGAGGCCTTGTTCTTGTTCCTACCAAGGAGCTGGCACGGCAAGCACAGTCCATGATTCAGCAGCTGGCTACCTACTGTGCTCGGGATGTCCGAGTGGCCAATGTCTCAGCTGCTGAAGACTCAGTCTCTCAGAGAGCTGTGCTGATGGAGAAGCCAGATGTGGTAGTAGGGACCCCATCTCGCATATTAAGCCACTTGCAGCAAGACAGCCTGAAACTTCGTGACTCCCTGGAGCTTTTGGTGGTGGACGAAGCTGACCTTCTTTTTTCCTTTGGCTTTGAAGAAGAGCTCAAGAGTCTCCTCTGTCACTTGCCCCGGATTTACCAGGCTTTTCTCATGTCAGCTACTTTTAACGAGGACGTACAAGCACTCAAGGAGCTGATATTACATAACCCGGTTACCCTTAAGTTACAGGAGTCCCAGCTGCCTGGGCCAGACCAGTTACAGCAGTTTCAGGTGGTCTGTGAGACTGAGGAAGACAAATTCCTCCTGCTGTATGCCCTGCTCAAGCTGTCATTGATTCGGGGCAAGTCTCTGCTCTTTGTCAACACTCTAGAACGGAGTTACCGGCTACGCCTGTTCTTGGAACAGTTCAGCATCCCCACCTGTGTGCTCAATGGAGAGCTTCCACTGCGCTCCAGGTGCCACATCATCTCACAGTTCAACCAAGGCTTCTACGACTGTGTCATAGCAACTGATGCTGAAGTCCTGGGGGCCCCAGTCAAGGGCAAGCGTCGGGGCCGAGGGCCCAAAGGGGACAAGGCCTCTGATCCGGAAGCAGGTGTGGCCCGGGGCATAGACTTCCACCATGTGTCTGCTGTGCTCAACTTTGATCTTCCCCCAACCCCTGAGGCCTACATCCATCGAGCTGGCAGGACAGCACGCGCTAACAACCCAGGCATAGTCTTAACCTTTGTGCTTCCCACGGAGCAGTTCCACTTAGGCAAGATTGAGGAGCTTCTCAGTGGAGAGAACAGGGGCCCCATTCTGCTCCCCTACCAGTTCCGGATGGAGGAGATCGAGGGCTTCCGCTATCGCTGCAGGGATGCCATGCGCTCAGTGACTAAGCAGGCCATTCGGGAGGCAAGATTGAAGGAGATCAAGGAAGAGCTTCTGCATTCTGAGAAGCTTAAGACATACTTTGAAGACAACCCTAGGGACCTCCAGCTGCTGCGGCATGACCTACCTTTGCACCCCGCAGTGGTGAAGCCCCACCTGGGCCATGTTCCTGACTACCTGGTTCCTCCTGCTCTCCGTGGCCTGGTGCGCCCTCACAAGAAGCGGAAGAAGCTGTCTTCCTCTTGTAGGAAGGCCAAGAGAGCAAAGTCCCAGAACCCACTGCGCAGCTTCAAGCACAAAGGAAAGAAATTCAGACCCACAGCCAAGCCCTCCTGA

DDX56, DDX21, DDX26, NOH61, DDX56, ATGD0497-10 µg, ATGD0497-20 µg, ATGD0497-50 µg, ATGD0497-100 µg, ATGD0497-250 µg, ATGD0497-500 µg, ATGD0497-1 mg, ATGD0497-10, ATGD0497-20, ATGD0497-50, ATGD0497-100, ATGD0497-250, ATGD0497-500, ATGD0497-1

Store the plasmid at -20C.

Lyophilized

Epigenomics (Transcription & Translation)

608023

NP_061955.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_019082.3

1644bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.