DEPDC5 Antilichaam/DEP domein-bevattende proteïne 5

The DEPDC5 gene encodes DEP domain-containing protein 5, a protein which contains a DEP (disheveled, EGL-10, pleckstrin) domain. This domain is known for its involvement in signal transduction and cellular communication, highlighting the importance of DEPDC5 in coordinating crucial cellular processes. DEP domain-containing protein 5 has been implicated in the regulation of cellular signaling pathways, including the mechanistic target of rapamycin complex 1 (mTORC1) pathway. By interacting with key proteins in this pathway, DEPDC5 plays a critical role in maintaining cellular homeostasis and growth. Mutations in the DEPDC5 gene have been linked to various neurological disorders, including familial focal epilepsy.

Western blot: 1:1000-1:2000, Flow cytometry: 1:25 per million cells in 0.1ml

O75140

Human

A portion of amino acids 1404-1432 from the human protein was used as the immunogen for the DEPDC5 antibody.

WB, FACS

Antigen affinity purified

Purified

This DEPDC5 antibody is available for research use only.

Aliquot the DEPDC5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

In 1X PBS, pH 7.4, with 0.09% sodium azide

The stated application concentrations are suggested starting points. Titration of the DEPDC5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Flow cytometry testing of fixed and permeabilized human HeLa cells with DEPDC5 antibody; Blue=isotype control, Green= DEPDC5 antibody.