Desmin Recombinant eiwit

Source : Escherichia Coli. Desmin Human Recombinant having a calculated molecular mass of 53,539 Dalton, showing a 55kDa band on SDS-page, pI-5.16. Desmin is a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.

Desmin||DES||CSM1||CSM2||CMD1I||FLJ12025||FLJ39719||FLJ41013||FLJ41793.

Greater than 95.0% as determined by SDS-PAGE.

Desmin was lyophilized from a 1mg/mL solution containing 30mM Tris-HCl pH 8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride.

Lyophilized Desmin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Desmin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

It is recommended to reconstitute the lyophilized Desmin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.