DFF40 Antilichaam

DFF40 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) . The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.

13368

O54788

NP_031885

Human, Mouse, Rat

Unconjugated

Primary Antibodies

Apoptosis

DFF40 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1204 - K562 Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Bovine: (94%)

DFF40 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

DFF40 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Dffb

Cat. No. 1205 - Jurkat Cell Lysate

Cat. No. 17-005 - Jurkat Cell Slide

DNA fragmentation factor, beta subunit

Mus musculus

Enari M, Sakahira H, Yokoyama H, et al. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998; 391:43-50.

Sakahira H, Enari M, and Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998; 391:96-99.

Halenbeck R, MacDonald Human, Ratoulston A, et al. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr. Biol. 1998; 8:537-40.

Liu X, Li P, Widlak P, at al. The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc. Natl. Acad. Sci. USA 1998; 95:8461-6.

DFF40 Antibody: CAD, CPAN, 40kDa, DFF40, Didff, 5730477D02Rik, Cad, DNA fragmentation factor subunit beta, Caspase-activated deoxyribonuclease, CAD

ELISA, WB, ICC, IF

160948620

Liquid