DFFA Antibody, KO gevalideerd

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

1676

O00273

Human, Mouse, Rat

Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFFA (NP_004392.1) .

Unconjugated

Primary Antibodies

Antibody is Knockout validated.

Apoptosis, Cell Cycle

Affinity purification

Jurkat

Batch dependent

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

None

Blue Ice

Store at -20˚ C. Avoid freeze / thaw cycles.

Observed: 35kDa, 45kDa

IgG

DFFA

H460

HepG2

Mouse brain

Rat brain

DNA fragmentation factor, 45kDa, alpha polypeptide

Homo sapiens

DFF45 Antibody: DFF1, ICAD, DFF-45, DFF1, DFF45, H13, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit

WB, IF

Liquid