DFFB Antilichaam - middengebied: Biotine (ARP52046 P050-Biotine)

DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)

CAD, CPAN, DFF2, DFF40, DFF-40

1677

O76075

NP_004393

Human

The immunogen is a synthetic peptide directed towards the middle region of human DFFB

DFFB is a nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. DFFB degrades naked DNA and induces apoptotic morphology.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

DFFA; UBC; NUBP1; MARCKS; APP; CAND1; COPS5; CUL1; CUL2; CUL5; NEDD8; CIDEB; DFFB; TOP2A

Biotin

Polyclonal Antibody

WB

Affinity Purified

0.5 mg/ml

Human: 100%

Liquid. Purified antibody supplied in 1x PBS buffer.

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

39kDa

Neimanis, S., (2007) J. Biol. Chem. 282 (49), 35821-35830

Wet Ice

338

DFFB

DFFB

Rabbit

DNA fragmentation factor subunit beta

NM_004402

EYFYGLLFTSENLKLVHIVCHKKTTHKLNCDPSRIYKPQTRLKRKQPVRK

Beta