DNA-RNA Hybride Antilichaam

DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. They are shown to be resistant to the activity of DNA methyltransferases. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2. Clone S9.6 bound the DNA-RNA heteropolymer and poly (I) -poly (dC) equally, but 100-fold higher levels of poly (A) -poly (dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986) . J. Immunol Methods. 89 (1) :123-130) .

Chromatin immunoprecipitation: per published data, Flow cytometry: per published data, Immunocytochemistry: per published data, Immunofluorescence: per published data

Human

A DNA-RNA heteropolymer duplex prepared by transcription of phi X174 single-stranded DNA with DNA-dependent RNA polymerase was used as the immunogen for the DNA-RNA Hybrid antibody.

S9.6

ChIP, FACS, ICC, IF

Protein G affinity

Purified

This DNA-RNA Hybrid antibody is available for research use only.

Aliquot the DNA-RNA Hybrid antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the DNA-RNA Hybrid antibody should be determined by the researcher.

Nucleus

SDS-PAGE analysis of purified, BSA-free DNA-RNA Hybrid antibody (clone S9.6) as confirmation of integrity and purity.