DNAJB8 cDNA

DNAJB8, also known as dnaJ homolog subfamily B member 8, is superior suppressors of aggregation and toxicity of disease-associated polyglutamine proteins. DNAJB8 is (de) acetylated at two conserved C-terminal lysines that are not involved in substrate binding, but do play a role in suppressing protein aggregation.

DJ6

3q21.3

Human

PATGen (puc19-derived cloning vector)

ATGGCTAACT ACTACGAAGT GCTGGGCGTG CAGGCCAGCG CTTCCCCGGA GGACATCAAG AAAGCCTACC GCAAGCTGGC CCTTCGTTGG CACCCCGACA AGAACCCTGA CAATAAGGAG GAGGCGGAGA AGAAGTTCAA GCTGGTGTCT GAGGCCTATG AGGTTCTGTC TGACTCCAAG AAACGCTCCC TGTATGACCG TGCTGGCTGT GACAGCTGGC GGGCTGGTGG CGGGGCCAGC ACGCCCTACC ACAGCCCCTT CGACACCGGC TACACCTTCC GTAACCCTGA GGACATCTTC CGGGAGTTTT TCGGTGGCCT GGACCCTTTC TCCTTTGAGT TCTGGGACAG CCCATTCAAT AGTGACCGTG GTGGCCGGGG CCATGGCCTG AGGGGGGCCT TCTCGGCAGG CTTTGGAGAA TTTCCGGCCT TCATGGAGGC CTTCTCATCC TTCAACATGC TGGGCTGCAG CGGGGGCAGC CACACCACCT TCTCATCCAC CTCCTTCGGG GGCTCCAGTT CTGGCAGCTC GGGGTTCAAG TCGGTGATGT CGTCCACCGA GATGATCAAT GGCCACAAGG TCACCACCAA GCGCATCGTG GAGAACGGGC AGGAGCGCGT GGAGGTGGAG GAAGACGGGC AGCTCAAGTC GGTGACTGTG AACGGCAAGG AGCAGCTCAA ATGGATGGAC AGCAAGTAG

DNAJB8, DJ6, DNAJB8, ATGD0086-10 µg, ATGD0086-20 µg, ATGD0086-50 µg, ATGD0086-100 µg, ATGD0086-250 µg, ATGD0086-500 µg, ATGD0086-1 mg, ATGD0086-10, ATGD0086-20, ATGD0086-50, ATGD0086-100, ATGD0086-250, ATGD0086-500, ATGD0086-1

Store the plasmid at -20C.

Lyophilized

Heat Shock Proteins

611337

NP_699161.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_153330.2

699bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.