DNMT3B Antilichaam - middelste regio : HRP (ARP49124_P050-HRP)

DNA (cytosine-5-) -methyltransferase 3 beta

ICF, ICF1, M.HsaIIIB

1789

Q9UBC3-3

NP_787045

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

The immunogen is a synthetic peptide directed towards the middle region of human DNMT3B

DNMT3B is required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. DNMT3B may preferentially methylate nucleosomal DNA within the nucleosome core region. DNMT3B may function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. DNMT3B seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, DNMT3B is involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

RBBP7; RBBP4; HDAC2; HDAC1; CHD4; MTA2; MTA1; POLR2H; POLR2C; POLR2A; DNMT1; LEO1; CDC73; PAF1; RTF1; CTR9; NRIP1; EZH2; DNMT3L; SUZ12; SIRT1; EED; UBC; EPHB1; CREB1; CBX5; TRIM28; ZFP57; UHRF2; UHRF1; MAP1LC3B; UBE2W; PLEKHJ1; DUSP23; CMTM6; PAM16; TSC22

HRP: Horseradish Peroxidase

Polyclonal Antibody

WB

Affinity Purified

DNMT3B is supported by BioGPS gene expression data to be expressed in OVCAR3

0.5 mg/ml

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

86kDa

Boland, M.J. (2008) J. Mol. Biol. 379 (3), 492-504

Wet Ice

770

DNMT3B

DNMT3B

Rabbit

DNA (cytosine-5) -methyltransferase 3B

NM_175849

GTGRLFFEFYHLLNYSRPKEGDDRPFFWMFENVVAMKVGDKRDISRFLEC