Dopamine-ELISA [CE]

Dopamine is extracted by using a cis-diol-specific affinity gel, acylated and then derivatized enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte concentrations compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The

HRP

ELISA

EDTA Plasma, Urine

300 µL for Plasma /10 µL for Urine

75 -33333 pg/ml for Plasma / 4.8 - 2000 ng/ml for Urine

49 pg/mL for Plasma / 2,5 ng/mL for Urine

ELISA

60 Weeks

https://cdn.gentaur.com/products/22/69ae95b01125b5183b569bdb/ifu/kapl10-0300.pdf

4,5 h with shaking 600 rpm

Biogenic Amines and Neurosciences

Dopamine

2 levels