DR1 cDNA

DR1, also known as NC2-beta, is a TBP (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. By selectively repressing polymerases II and III, DR1 may shift the physiological balance of transcriptional output in favor of polymerase I.

NC2, NC2-BETA

1p22.1

Human

PATGen (puc19-derived cloning vector)

ATGGCTTCCT CGTCTGGCAA CGATGATGAT CTCACTATCC CCAGAGCTGC TATCAATAAA ATGATCAAAG AGACTCTTCC TAATGTCCGG GTGGCCAACG ATGCTCGAGA GCTGGTGGTG AACTGCTGCA CTGAATTCAT TCACCTTATA TCTTCTGAAG CCAATGAGAT TTGTAACAAA TCGGAAAAGA AGACCATCTC ACCAGAGCAT GTCATACAAG CACTAGAAAG TTTGGGATTT GGCTCTTACA TCAGTGAAGT AAAAGAAGTC TTGCAAGAGT GTAAAACAGT AGCATTAAAA AGAAGAAAGG CCAGTTCTCG TTTGGAAAAC CTTGGCATTC CTGAAGAAGA GTTATTGAGA CAGCAACAAG AATTATTTGC AAAAGCTAGA CAGCAACAAG CAGAATTGGC CCAACAGGAA TGGCTTCAAA TGCAGCAAGC TGCCCAACAA GCCCAGCTTG CTGCTGCCTC AGCCAGTGCA TCTAATCAGG CGGGATCTTC TCAGGATGAA GAAGATGATG ATGATATCTG A

DR1, NC2, NC2-BETA, DR1, ATGD0030-10 µg, ATGD0030-20 µg, ATGD0030-50 µg, ATGD0030-100 µg, ATGD0030-250 µg, ATGD0030-500 µg, ATGD0030-1 mg, ATGD0030-10, ATGD0030-20, ATGD0030-50, ATGD0030-100, ATGD0030-250, ATGD0030-500, ATGD0030-1

Store the plasmid at -20C.

Lyophilized

Epigenomics (Transcription & Translation)

601482

NP_001929.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_001938.2

531bp

This shuttle vector contains the complete ORF. It is inseted Nde I to BamH I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.