DR4 Antilichaam

DR4 Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4) . The ligand for this novel death receptor has been identified and termed TRAIL, which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1) . DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-κ B activation in many tissues and cells.

8797

O00220

AAC51226

Human, Mouse, Rat

1167

Unconjugated

Primary Antibodies

KO Validation (Figure 1) : Anti-DR4 antibodies (1167) specificity was further verified by DR4 specific knockout cell lysate. DR4 signal was detected in wild type cells, but not in the DR4 knockout cells. KO Validation1167) Independent Antibody Validation in Cell lines (Figure 2) shows similar DR4 expression profile in human cell lines detected by two independent anti-DR4 antibodies that recognize different epitopes, 1139 against C-terminus domain and 1167 against the N-terminus domain. DR4 proteins are detected in all the tested cell lines except CaCo-2 at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines1139 1167 KD validation (Figure 6, 10) : Anti-DR4 antibody specificity was further verified by DR4 specific siRNA knockdown. DR4 signal in SW480 and HeLa cells transfected with DR4 siRNAs was disrupted in comparison with that in cells transfected with control siRNAs. KD validation

Apoptosis

DR4 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1202 - A431 Cell Lysate

1 mg/ml

DR4 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

DR4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

DR4 antibody has no cross reaction to DR5.

Human DR4 has only 1 isoform (468aa, 50kD) .

TNFRSF10A

Cat. No. 1203 - A549 Cell Lysate

Cat. No. 1224 - Daudi Cell Lysate

Cat. No. 1201 - HeLa Cell Lysate

Tumor necrosis factor receptor superfamily, member 10a

Homo sapiens

Pan et al. Science 1997;276:111-3

Wiley et al. Immunity 1995; 3:673-82

Pitti et al. J. Biol. Chem. 1996;271:12687-90

Schneider et al. Immunity 1997; 7:831-6

18193086

25032854

18511292

27827395

16007131

17470478

29020632

20562908

24980819

18594532

21941003

18599488

17212362

21835222

17522150

28529596

22784708

27406983

21372226

19414356

DR4 Antibody: DR4, APO2, CD261, TRAILR1, TRAILR-1, DR4, Tumor necrosis factor receptor superfamily member 10A, Death receptor 4, TRAIL receptor 1

ELISA, WB, IF, ICC

1945072

Liquid