DRAM-antilichaam

DRAM Antibody: Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.

55332

Q8N682

AAH18435

Human, Mouse, Rat

4033

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar DRAM expression profile in human, mouse and rat cell lines detected by two independent anti-DRAM antibodies that recognize different epitopes, 4033 against C-terminus domain and 4035 against N-terminus domain. DRAM proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines4033 4035 Induced Expression Validation (Figure 5) : DRAM expression detected by anit-DRAM antibodies (4033) was up-regulated by CQ treatment in WT neural precursor cells. Induced Expression Validation4033 Regulated expression validation (Figure 6) : DRAM expression detected by anit-DRAM antibodies (4033) was down-regulated by in WT neural precursor cells. Regulated expression validation4033

Apoptosis, Autophagy, Cancer

DRAM Antibody is affinity chromatography purified via peptide column.

Cat. No. 1210 - HEK293 Cell Lysate

1 mg/mL

DRAM Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

DRAM antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human DRAM has 2 isoforms, including isoform 1 (238aa, 26kD) and isoform 2 (128aa, 14kD) . Mouse DRAM also has 2 isoforms, including isoform 1 (238aa, 26kD) and isoform 2 (132aa, 15kD) . Rat DRAM has only one isoform (238aa, 26kD) . 4033 can detect human, mouse and rat isoforms.

DRAM1

Cat. No. 1304 - Human Liver Tissue Lysate

Cat. No. 10-201 - Human Liver Tissue Slide

Damage-regulated autophagy modulator

Homo sapiens

Crighton, et al. Cell 2006; 126:121-34.

Gozuacik and Kimchi. Oncogene. 2004; 23:2891-906.

Crighton, et al. Autophagy 2007; 3:72-4.

20123985

21720543

DRAM Antibody: DRAM, DRAM, DNA damage-regulated autophagy modulator protein 1, Damage-regulated autophagy modulator

ELISA, WB, IHC-P, IF

22450862

Liquid