E. coli-longfactor 4 Proteïne, zijn tag (active enzym)

Elongation Factor 4 (EF4), also known as ​​LepA​​, is a highly conserved ​​GTPase translation factor​​ present across bacteria, mitochondria, and chloroplasts. It regulates protein synthesis by interacting with the ribosome during translation elongation. Unlike canonical elongation factors (e.g., EF-G) that promote forward translocation, EF4 catalyzes ​​back-translocation of tRNAs​​ on the ribosome, effectively acting as a molecular ’reverse gear‘ to reposition tRNAs and mRNA. This unique function allows EF4 to maintain translational fidelity under stress conditions (e.g., low pH or high ionic strength) by rescuing stalled ribosomes and correcting errors in protein synthesis.

Elongation Factor 4 (EF4), also known as ​​LepA​​, is a highly conserved ​​GTPase translation factor​​ present across bacteria, mitochondria, and chloroplasts. It regulates protein synthesis by interacting with the ribosome during translation elongation. Unlike canonical elongation factors (e.g., EF-G) that promote forward translocation, EF4 catalyzes ​​back-translocation of tRNAs​​ on the ribosome, effectively acting as a molecular ’reverse gear‘ to reposition tRNAs and mRNA. This unique function allows EF4 to maintain translational fidelity under stress conditions (e.g., low pH or high ionic strength) by rescuing stalled ribosomes and correcting errors in protein synthesis.

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 68.5 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

Lys 2 - Lys 599

Elongation Factor 4

Unconjugated

N-10xHis

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

90%

Liquid

PBS, 0.5 M NaCl, 1 M Arginine, pH7.4

68.5 kDa

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Dry ice

-70°C

50ug*1

E. coli

E. coli

C4ZYJ2

Glycerol